Evercode™ WT

Evercode™ Whole Transcriptome
Single Cell for Any Lab

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WT Mini

10k
Cells
12
Samples
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WT

Comprehensive gene profiling across samples, replicates, or timepoints for most studies.

100k
Cells
48
Samples

WT Mega

1M
Cells
96
Samples
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Scalable Single Cell Sequencing Without the Instrument

Single Cell RNA-Seq For Today's Experiments

Evercode Whole Transcriptome products are enabling scientists to push scRNA-Seq past the limitations of previous technologies to detect more transcripts and genes per cell, enabling a deeper understanding of biological function.
Gene Detection

Unmatched Gene Detection

More genes and transcripts detected per cell provide comprehensive results over conventional approaches.

Industry leading sensitivity

As experiments scale, the unmatched gene detection of smaller scales consistently applies within the Evercode line.


Gene Detection

All Signal, No Noise

Multiplets are a nuisance of single cell RNA-Seq approaches that complicate data analysis. When the cell is the reaction vessel, issues related to multiple cells in a droplet are eliminated and it shows up in data cleanliness.

Lower Multiplet Rates Than Droplet Based Approaches

In a human-mouse species mixing experiment utilizing the WT, we detect only 1.7% multiplets - much lower than droplet-based approaches.


All Signal, No Noise
No Instrument Required

No Instrument Required

Evercode combinatorial barcoding is performed on your lab bench – all that’s needed are a centrifuge, thermal cycler, and some pipettes. The single cell or single nuclei libraries are ready for loading on an Illumina sequencer.

A Simpler Workflow
Through Fixation

Fix cells or nuclei to lock in the biology until your experiment is ready. In timecourse studies, avoid uncertainty and remove batch bias by running samples collected on different days together.

Fixed samples stay ready for at least 6 months

Fixation locks in the biology to provide workflow flexibility. We checked a freshly prepared sample against the same sample stored for 6 months to prove stability of the fixation and reproducibility of the assay.


A Simpler Workflow Through Fixation

Single Cell Genomics –
Cells and Nuclei

Difficult samples or complex research questions often require a single nuclei approach. The Evercode technology works with both single nuclei sequencing (snRNA-Seq) and single cell sequencing (scRNA-Seq).

Single Cell Genomics – Cells and Nuclei

Testimionial

Combinatorial barcoding finally made it feasible to analyze a large number of samples with low reagent and time costs, and the data quality is top-notch. We used Parse Biosciences' kits to study the effects of BSL-3 pathogens on airway epithelia at single cell resolution. Moreover, Parse provided amazing customer support.

Alejandro Pezzulo Colmenares, MD
University of Iowa
The Parse Biosciences Workflow

From Bench
to Insight

The Evercode Whole Transcriptome technology provides the reagents, software, and accessibility to pursue difficult research questions. This approach first fixes a single cell or nuclei suspension. Once fixed, the barcoding and library prep process outputs libraries ready for any Illumina sequencer. Following sequencing, our software converts data into single cell insights.
1
Parse Biosciences Process - Step 1

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

2
Parse Biosciences Process - Step 2

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through four split-pool combinatorial barcoding steps. The kit proceeds to a standard library preparation to generate sequencing-ready molecules.
3
Parse Biosciences Process - Step 3

Sequencing

The resulting libraries are sequenced by NGS.
4
Parse Biosciences Process - Step 4

Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Parse Biosciences Brochure

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Read more about the technology with a deeper dive into the technical details.

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Explore the Sample Data

Review the Evercode Whole Transcriptome data from a species mixing experiment, immune profiling in PBMCs, or nuclei from embryonic mouse brain.

Explore sample data

Support

Information and insight for the entire range of Parse Biosciences products.

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Advancing Research for 400+ Labs