Evercode™ WT v3
SINGLE CELL WHOLE TRANSCRIPTOME

More scalable, with deeper insights

Evercode™ Whole Transcriptome products are enabling scientists to push scRNA-seq past the limitations of previous technologies to scale up the samples and cells per experiment while also detecting more genes per cell. And all with no instrument purchase required.

See example datasets

100K

Cells

48

Samples

Evercode™ WT Mini

Up to 10K cells, 1-12 samples

Evercode™ WT

10K-100K cells, 1-48 samples

Evercode™ WT Mega

100K-1M cells, 1-384 samples

Evercode™ WT Penta

1M-5M cells, 1-384 samples

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

Achieve your single cell ambitions

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Better detect lowly expressed genes and avoid ambient RNA common in droplet-based single cell sequencing.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Learn more about the technology

Higher sensitivity with Evercode WT

Evercode WT v2 detected an average of 84% more genes than the Chromium Next GEM Single Cell 3’ Kit v3.1 at the common read depth target of 20,000 reads/cell in mouse brain.

See the 10x comparison study

Transition seamlessly to Evercode

Merge past and present research efforts, maintaining continuity in your data while exploring new frontiers.

Integrates with existing data

Single cell data from 17k mouse brain nuclei analyzed by Evercode WT v2 and Chromium Next GEM Single Cell 3' v3.1 were integrated, clustered, and annotated - identifying the same cell types.

Compare Evercode™ WT v2 with droplet-based technology

Parse Biosciences Evercode™ WT v2 combinatorial barcoding technology was compared with the droplet-based 10x Genomics™ Chromium™ Next GEM Single Cell 3’ Kit v3.1 with mouse brain nuclei. This heterogeneous sample type has been the focus of an array of cell atlas projects and individual researchers.

View comparison results

Comparison study design

Two E18 embryonic C57/BI6 samples were collected, sagittally dissected into 2 halves, and flash frozen by a third-party tissue vendor. Half of each brain was shipped to a 10x Genomics™ Certified Service Provider, and they isolated nuclei with the Chromium™ Nuclei Isolation Kit and created sequencing libraries with the Chromium™ Next GEM Single Cell 3’ GEM Library & Gel Bead Kit v3.1. The other halves of each brain were processed by Parse Biosciences for nuclei isolation with a dounce homogenizer, fixation with Evercode™ Nuclei Fixation v2, and library preparation with Evercode™ WT v2. Sequencing libraries from each technology were sequenced by a third party. The sequencing data were analyzed with each manufacturer’s data analysis pipeline.

Boost the signal, reduce the noise

Multiplets are a nuisance of scRNA-seq approaches that complicate data analysis. When the cell is the reaction vessel, issues related to multiple cells in a droplet are eliminated, thus improving data cleanliness.

Lower multiplet rates than  droplet-based approaches

In a human-mouse species mixing experiment using the WT, we detected only 2.3% multiplets—much lower than droplet-based approaches.

We detected rare cell types with Evercode’s higher sensitivity

Yi Xie

Duke-NUS Medical School

Enrico Petretto

Duke-NUS Medical School

Jacques Behmoaras

Duke-NUS Medical School

See 10x comparison study

The Parse Biosciences workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

Sequencing

The resulting libraries are sequenced by NGS.

Data Analysis

Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.