Evercode™ WT v2

Resolve More Biology

Connect with a single cell expert

WT Mini

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Comprehensive gene profiling across samples, replicates, or timepoints for most studies.


WT Mega

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Scalable Single Cell Sequencing Without the Instrument

Easy to Get Started

Evercode™ Whole Transcriptome products are enabling scientists to push scRNA-Seq past the limitations of previous technologies to detect more genes per cell, enabling a deeper understanding of biological function – with no capital expense of an instrument required. This also means no more rushing samples between labs or scheduling time on a shared instrument.
Gene Detection

Superior Gene Detection

More genes detected per cell provide comprehensive results over conventional approaches.

Industry leading sensitivity

Sequencing saturation plot generated from mouse liver samples prepared using Evercode™ WT v2.

Gene Detection

Unbiased Gene Expression

Combine previous experimental data with full compatibility of results. We didn’t take any shortcuts or introduce bias when developing the top performing single cell RNA-Seq assay.

Unbiased Gene Expression
Consistent Results
Robust Assay Performance

PBMC samples from four individual donors were prepared together to illustrate the reproducibility of the assay. The median and distribution of genes is consistent across samples.

Consistent Results Every Time

Improvements in single cell technology have created a more predictable single cell RNA-Seq assay. Reduce sample dropout across samples and sample types - in both the single cell and single nuclei assays.

All Signal, No Noise

Multiplets are a nuisance of single cell RNA-Seq approaches that complicate data analysis. When the cell is the reaction vessel, issues related to multiple cells in a droplet are eliminated and it shows up in data cleanliness.

Lower Multiplet Rates Than Droplet Based Approaches

In a human-mouse species mixing experiment utilizing the WT, we detect only 1.7% multiplets - much lower than droplet-based approaches.

All Signal, No Noise

A Simpler Workflow
Through Fixation

Fix cells or nuclei to lock in the biology until your experiment is ready. In timecourse studies, avoid uncertainty and remove batch bias by running samples collected on different days together.

Fixed samples stay ready for at least 6 months

Fixation locks in the biology to provide workflow flexibility. We checked a freshly prepared sample against the same sample stored for 6 months to prove stability of the fixation and reproducibility of the assay (Evercode™ WT v1 results shown).

A Simpler Workflow Through Fixation

Single Cell Genomics –
Cells and Nuclei

Difficult samples or complex research questions often require a single nuclei approach. The Evercode technology works with both single nuclei sequencing (snRNA-Seq) and single cell sequencing (scRNA-Seq).

Single Cell Genomics – Cells and Nuclei


Combinatorial barcoding finally made it feasible to analyze a large number of samples with low reagent and time costs, and the data quality is top-notch. We used Parse Biosciences' kits to study the effects of BSL-3 pathogens on airway epithelia at single cell resolution. Moreover, Parse provided amazing customer support.

Alejandro Pezzulo Colmenares, MD
University of Iowa
The Parse Biosciences Workflow

From Bench
to Insight

The Evercode Whole Transcriptome technology provides the reagents, software, and accessibility to pursue difficult research questions. This approach first fixes a single cell or nuclei suspension. Once fixed, the barcoding and library prep process outputs libraries ready for any Illumina sequencer. Following sequencing, our software converts data into single cell insights.
Parse Biosciences Process - Step 1


Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Parse Biosciences Process - Step 2

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through four split-pool combinatorial barcoding steps. The kit proceeds to a standard library preparation to generate sequencing-ready molecules.
Parse Biosciences Process - Step 3


The resulting libraries are sequenced by NGS.
Parse Biosciences Process - Step 4

Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Parse Biosciences Brochure

Get the Brochure

Read more about the technology with a deeper dive into the technical details.

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Explore the Sample Data

Review a collection of datasets that compare differences between Parse Biosciences v1 and v2 chemistries.

Explore sample data


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