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Technology

Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

Technology Overview


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Explore our collection of resources to learn more about technology and its applications from leading researchers

Resources Overview

Company

Providing researchers single cell sequencing with unprecedented scale and ease

About Parse

Evercode™ WT Mega v3
SINGLE CELL WHOLE TRANSCRIPTOME

Scale your projects to millions of cells

1M

Cells

96

Samples

Run up to 1 million cells and 96 biological samples or conditions in a single experiment. Scale exponentially with Evercode combinatorial barcoding.

See example datasets

1M

Cells

96

Samples

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

A simpler way to scale your single cell projects

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Better detect lowly expressed genes and avoid ambient RNA common in droplet-based single cell sequencing.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Learn more about the technology

Higher Sensitivity

Evercode WT v2 detected an average of 84% more genes than the Chromium Next GEM Single Cell 3’ Kit v3.1 at the common read depth target of 20,000 reads/cell in mouse brain.

See the 10x comparison study

Evercode™ delivers more results

Compared with the leading droplet-based technologies, Evercode™ requires less reagents and time.

Fix now, run later

Fix cells or nuclei as they're available to lock in the biology. After a 1‐hour fixation, samples are stable for 6 months. Whether working on a timecourse study, sharing samples between laboratories, or segregating sample preparation from core lab space, fixation provides the flexibility to work the way you desire.

Unleash high throughput

Evercode WT Mega enables the analysis of up to a million cells or 96 samples with a 2-day workflow. Boost your research depth and breadth effortlessly, capturing intricate biological heterogeneity and boosting statistical power with ease.

From Samples to Sequencer in 2 days

We detected rare cell types with Evercode’s higher sensitivity


Yi Xie

Duke-NUS Medical School

Enrico Petretto

Duke-NUS Medical School

Jacques Behmoaras

Duke-NUS Medical School

The Parse Biosciences workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.

1
Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

2
Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

3
Sequencing

The resulting libraries are sequenced by NGS.

4
Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

WT Mega: Resources and support

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Read more about the technology and dive deeper into the technical details.

Support

More information and literature across the entire range of Parse Biosciences products.

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Reach out for a quote or for help planning your next experiment.