Cells
Samples
Explore a wide variety of adaptive immunity applications through Evercode™ BCR. Without needing to purchase proprietary hardware, your lab can delve into the immune response, detecting paired heavy-light chains to characterize the immune repertoire complexity.
Explore datasetsCells
Samples
Up to 10K cells, 1-12 samples
10K-100K cells, 1-48 samples
100K-1M cells, 1-96 samples
Up to 10K cells, 1-12 samples
10K-100K cells, 1-48 samples
100K-1M cells, 1-96 samples
Reveal the full complexity of the immune repertoire with sensitive detection of BCRs. Measure transcriptome-wide gene expression together with full-length paired heavy and light sequences and isotypes in the same cells.
Scale up your experiments to capture the full complexity of the immune repertoire.
In this study, over a million isolated human B cells were profiled from 24 donors including individuals with Type-1 Diabetes, Multiple Sclerosis, Rheumatoid Arthritis, Crohn's, and Celiac disease.
Revealing the complexity of the immune repertoire requires sensitive detection of BCRs along with whole transcriptome profiles.
In an experiment comparing T1D and control donors, Evercode BCR captured both heavy and light chains with 87-89% BCR pairing efficiency
The Evercode BCR kit generates the most comprehensive immune repertoire detection from a single experiment to date. Over 900,000 unique paired clonotypes were identified across the 24 donors with the vast majority being classified as unique clonotypes.
Stimulated B cells can differentiate and undergo isotype switching resulting in both memory B cells and antibody secreting plasma cells. Isotype switching retains the variable region, but switches the constant region isotype resulting in antibodies with different biological responses. Evercode Fixation preserves fragile cell types like plasma cells, enabling deep profiling of the clonal diversity and providing insight into patient specific immune responses.
The Evercode™ BCR solution provides the reagents, software, and accessibility to pursue difficult research questions.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
The resulting libraries are sequenced by NGS.
Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology and dive deeper into the technical details.
More information and literature across the entire range of Parse Biosciences products.
Reach out for a quote or for help planning your next experiment.
