EVERCODE™ BCR
SINGLE CELL PAIRED BCR + WHOLE TRANSCRIPTOME

More insights with more scalable single cell BCR profiling

Up to 1M

Cells

Up to 96

Samples

Explore a wide variety of adaptive immunity applications through Evercode™ BCR. Without needing to purchase proprietary hardware, your lab can delve into the immune response, detecting paired heavy-light chains to characterize the immune repertoire complexity.

Explore datasets

Up to 1M

Cells

Up to 96

Samples

Evercode™ BCR Mini

Up to 10K cells, 1-12 samples

Evercode™ BCR

10K-100K cells, 1-48 samples

Evercode™ BCR Mega

100K-1M cells, 1-96 samples

Evercode™ BCR Mini

Up to 10K cells, 1-12 samples

Evercode™ BCR

10K-100K cells, 1-48 samples

Evercode™ BCR Mega

100K-1M cells, 1-96 samples

Capture full length BCR sequences and gene expression together at unprecedented scale

Reveal the full complexity of the immune repertoire with sensitive detection of BCRs. Measure transcriptome-wide gene expression together with full-length paired heavy and light sequences and isotypes in the same cells.

1M B cells in a single experiment

Scale up your experiments to capture the full complexity of the immune repertoire.

In this study, over a million isolated human B cells were profiled from 24 donors including individuals with Type-1 Diabetes, Multiple Sclerosis, Rheumatoid Arthritis, Crohn's, and Celiac disease.

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

A simpler way to scale your single cell immune profiling

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free workflow that converts the cell or nucleus into an individual reaction compartment. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Evercode provides sensitive detection of full-length BCRs and isotypes, along with whole transcriptome profiles.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Sensitive heavy and light chain detection at scale

Revealing the complexity of the immune repertoire requires sensitive detection of BCRs along with whole transcriptome profiles.

In an experiment comparing T1D and control donors, Evercode BCR captured both heavy and light chains with 87-89% BCR pairing efficiency

Detect More Clonotypes Per Experiment

The Evercode BCR kit generates the most comprehensive immune repertoire detection from a single experiment to date. Over 900,000 unique paired clonotypes were identified across the 24 donors with the vast majority being classified as unique clonotypes.

Insights into isotype switching

Stimulated B cells can differentiate and undergo isotype switching resulting in both memory B cells and antibody secreting plasma cells. Isotype switching retains the variable region, but switches the constant region isotype resulting in antibodies with different biological responses. Evercode Fixation preserves fragile cell types like plasma cells, enabling deep profiling of the clonal diversity and providing insight into patient specific immune responses.

The Evercode™ BCR workflow

The Evercode™ BCR solution provides the reagents, software, and accessibility to pursue difficult research questions.

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

Sequencing

The resulting libraries are sequenced by NGS.

Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

Resources

Get the Brochure

Read more about the technology and dive deeper into the technical details.

Download the brochure

Support

More information and literature across the entire range of Parse Biosciences products.

Support Suite

We're your partners in single cell

Reach out for a quote or for help planning your next experiment.

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode + Integra

Resources

Trailmaker

Company

Recent News

In a Key Win for Parse, Patent Trial and Appeal Board Invalidates 10x Genomics Patent Claims

Kaitie Kramer Joins Parse Biosciences as Vice President of Marketing

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode + Integra

Resources

Trailmaker

Company

Recent News

In a Key Win for Parse, Patent Trial and Appeal Board Invalidates 10x Genomics Patent Claims

Kaitie Kramer Joins Parse Biosciences as Vice President of Marketing

More insights with more scalable single cell BCR profiling

Up to 1M

Up to 96

Up to 1M

Up to 96

Evercode™ BCR Mini

Evercode™ BCR

Evercode™ BCR Mega

Evercode™ BCR Mini

Evercode™ BCR

Evercode™ BCR Mega

Capture full length BCR sequences and gene expression together at unprecedented scale

1M B cells in a single experiment

A simpler way to scale your single cell immune profiling

Exponentially scalable

No instrument required

Unmatched data quality

Works with fixed cells and nuclei

Sensitive heavy and light chain detection at scale

Detect More Clonotypes Per Experiment

Insights into isotype switching

The Evercode™ BCR workflow

Fixation

Barcoding & Library Prep

Sequencing

Data Analysis

Resources

Get the Brochure

Support

We're your partners in single cell