Single cell pooled CRISPR screens enable analysis of complex phenotypes by linking individual gene perturbations with gene expression profiles. This approach has been used for target validation in drug discovery, to understand differences in treatment responses, and map pathways involved in cell differentiation. Wider adoption of this approach has been constrained by the feasibility and high cost of processing sufficient cells for libraries containing more than a few hundred guide RNAs (sgRNAs). CRISPR Detect brings the scalability of Evercode™ technology to single cell CRISPR screens, making genome-wide studies practical.
Scale easily with CRISPR Detect and Evercode™ Whole Transcriptome kits.
The published number of 10x Genomics Chromium Next GEM Single 3’ Kit lanes and projected Evercode™ Whole Transcriptome kits to obtain the same number of cells.
Avoid wasted sequencing and underpowered studies.
LN18 cells were prepared to only express one sgRNA then analyzed with Evercode™ WT Mega v2 and CRISPR Detect.
CRISPR Detect is a powerful addition to Evercode™ Whole Transcriptome capabilities and seamlessly integrates into the same workflow.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through split-pool combinatorial barcoding, then proceed for library preparation.
CRISPR Detect pairs with Evercode™ Whole Transcriptome to enrich sgRNA sequences.
Evercode™ Whole Transcriptome and CRISPR Detect libraries are sequenced together.
A computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology with a deeper dive into the technical details.
Sequence up to 1 million cells, no instrument required.
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