Explore a wide variety of adaptive immunity applications through Evercode™ TCR. Without needing to purchase proprietary hardware, your lab can delve into the immune response, detecting paired alpha-beta chains to characterize the immune repertoire complexity.Explore example datasets
Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.
Isolated T-cells from peripheral blood mononuclear cells (PBMCs) were either directly profiled (Primary) or placed in culture for 3 days and activated with CD3/CD28 beads and IL-2 (Activated).
The Evercode™ TCR kit generates the most comprehensive profile of the immune repertoire. Nearly 500,000 unique beta chain clonotypes were identified across 8 donors, with the vast majority being classified as rare clonotypes.
University of Pennsylvania
The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
The resulting libraries are sequenced by NGS.
Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology and dive deeper into the technical details.
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