Technology

Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

Technology Overview


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Explore our collection of resources to learn more about technology and its applications from leading researchers

Resources Overview

Company

Providing researchers single cell sequencing with unprecedented scale and ease

About Parse

Large-Scale Single Cell Services
GigaLab
10M+ Cells

For projects past the scale of a single kit. Send us your samples and we run the whole thing end to end, from barcoding through sequencing to analysis-ready data.

INTRODUCING EVERCODE MAX WHOLE TRANSCRIPTOME

Maximize What Matters Most in Your Single Cell Study

Evercode MAX lets you build a kit around the number of samples and cells per sample you plan to analyze. Every kit delivers sensitive gene expression profiling in an instrument-free workflow, and unused library prep reactions can be saved for future experiments.

More of What You Want, Less of What You Spend

Go bigger with your existing budget – MAX enables profiling of up to 2.5x more cells across up to 2x more samples with the same instrument-free workflow and lower cost per sample and cell.

Discover More Biology With Evercode MAX

Evercode split-pool combinatorial barcoding enables unbiased whole transcriptome gene expression profiling of cells or nuclei without a specialized instrument. Evercode MAX WT is the next evolution of this technology, delivering the highest possible resolution to your single cell project.

Maximize Scale on
Your Terms

Evercode MAX WT kits are tailored to the sample count and cells per sample your study needs.

Maximize
Efficiency

Use Evercode MAX across single or multiple experiments while using only the library prep reactions needed for your biology.

Maximize
Data Quality

Highly sensitive gene expression profiling helps you detect lowly expressed genes and distinguish subtle cell states.

Maximize Flexibility
with Fixation

Evercode fixation lets you collect and store samples across timepoints and locations, then process them together on your schedule.

Configure MAX Around Your Study

Separate cell barcoding and library prep modules let you match the kit to your sample count and the cells you plan to analyze. Additional library prep reactions can be added when needed, and unused reactions can be saved for future experiments.

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Size Your Next Single Cell Experiment.

We’ll give you an instant quote on how much it may cost to run your experiment.

Total target cells240,000

Collect Across Timepoints and Locations

Evercode fixation lets you store cells or nuclei as samples become available and process them together later. This helps you coordinate collection across sites and timepoints, work with core facilities, and reduce technical variation associated with separate processing runs.

Low Doublet Rates

Low doublet rates help keep gene expression profiles attributable to individual cells or nuclei. Evercode uses each cell or nucleus as the reaction vessel during split-pool combinatorial barcoding, helping limit profiles that combine transcripts from multiple cells.

Doublet rates across the Evercode kit sizes tested. These results demonstrate Evercode platform performance rather than a Max-specific benchmark.

Higher Sensitivity With Evercode WT

Sensitive Evercode chemistry helps you detect lowly expressed genes and distinguish subtle cell states. Evercode MAX WT brings that sensitivity to a kit tailored to the samples and cells your study needs.

In human PBMCs at 40,000 reads per cell, Evercode detected an average of 27.5% more transcripts than Chromium GEM-X Single Cell 3’ Kit v4.

The Parse Biosciences Workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

Sequencing

The resulting libraries are sequenced by NGS.

Data Analysis

Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.

“We could essentially do two experiments for the price of one. That made the decision pretty easy.”
Nicholas Jendzjowsky, PhD
Assistant Professor-in-Residence, The Lundquist Institute

Frequently Asked Questions

What Samples Can I Use for Single Cell RNA Sequencing?

What Sequencing Depth Do I Need for Single Cell RNA Sequencing With Evercode MAX WT?

Which Evercode Product Supports Larger Single Cell RNA Sequencing Studies?

How Much Does Evercode MAX WT Cost?

What Is Evercode MAX WT?

Resources and Support

Get the Brochure

Read more about the technology and dive deeper into the technical details.

Build Your Next Single Cell Study

Evercode MAX WT lets you build a kit around the samples and cells per sample in your next study.
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