Comparison of Evercode™ WT v4 and Chromium™ Single Cell Gene Expression Flex v2 (Apex) Kit in Fixed Human PBMCs

Key Tech Note Takeaways

• Head-to-head comparison shows >4x higher overall cell retention.
• >60% higher median transcripts detected per cell including diverse RNA biotypes.
• Probe-based methods produce inconsistent measurements in comparison to RT-based methods.

Experimental Design

Frozen PBMCs from two donors were obtained from a third-party provider. Aliquots derived from each donor were distributed to separate laboratories for processing. One aliquot per donor was shipped to a 10x Genomics Certified Service Provider for cell preparation and library construction using the Chromium GEM-X Flex v2 (Apex) kit. Parallel aliquots were processed by Parse Biosciences using the Evercode WT v4 workflow. Sequencing libraries from both technologies were sequenced on the same sequencer model, and data were analyzed using each manufacturer’s respective analysis pipeline