Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

Technology Overview


Explore our collection of resources to learn more about technology and its applications from leading researchers

Resources Overview


Providing researchers single cell sequencing with unprecedented scale and ease

About Parse

Evercode™ WT Mini v3

Single cell without the instrument





The Evercode™ WT Mini is perfectly sized to begin exploring single cell transcriptomics and experience the quality of results possible with Parse. Generate proof-of-concept results before you scale up to the Evercode™ WT or Evercode™ WT Mega to delve deeper into your biology.

See example datasets






Get started with single cell

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Better detect lowly expressed genes and avoid ambient RNA common in droplet-based single cell sequencing.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Learn more about the technology

Higher sensitivity with Evercode

Evercode WT v2 detected an average of 84% more genes than the Chromium Next GEM Single Cell 3’ Kit v3.1 at the common read depth target of 20,000 reads/cell in mouse brain.

See the 10x comparison study

Start small.
Dream big.

The Evercode WT Mini Kit is your gateway to the world of single-cell transcriptomics. Lay the groundwork for comprehensive studies before scaling to the Evercode WT and Evercode WT Mega kits.

See the kit comparison study

Consistent quality at every scale

From the first experiment to large-scale projects, Evercode Whole Transcriptome ensures unwavering data quality. Begin your journey with peace of mind as data quality is unchanged as you scale.

Equivalent Cell Type Proportions

Relative abundance of each cell type for the available Evercode Whole Transcriptome v3 kits in identical human PBMC samples.

Lower multiplet rates than droplet-based approaches

In a human-mouse species mixing experiment using the WT, we detected
only 2.3% multiplets—much lower than droplet-based approaches.

We detected rare cell types with Evercode’s higher sensitivity

Yi Xie

Duke-NUS Medical School

Enrico Petretto

Duke-NUS Medical School

Jacques Behmoaras

Duke-NUS Medical School

The Parse Biosciences workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.


Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.


The resulting libraries are sequenced by NGS.

Data Analysis

Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.

WT Mini: Resources and support

Get the Brochure

Read more about the technology and dive deeper into the technical details.


More information and literature across the entire range of Parse Biosciences products.

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Reach out for a quote or for help planning your next experiment.