Connect T Cell Clonotypes to Cell State at Scale
Pair alpha-beta TCR CDR3 sequences with whole transcriptome gene expression from the same cells to explore T cell diversity, clonal expansion, and function, with the flexibility to profile up to 2 million cells and 96 samples.
More of What You Want, Less of What You Spend
Go bigger with your existing budget – MAX enables profiling of up to 2.5x more cells across up to 2x more samples with the same instrument-free workflow and lower cost per sample and cell.
Discover More Biology With Evercode MAX
Evercode split-pool combinatorial barcoding enables T cell receptor and whole transcriptome gene expression profiling without specialized instrumentation, with cell fixation to preserve biology before processing. Evercode MAX TCR is the next evolution of this technology, combining sensitive repertoire detection with flexible study scale for a deeper view of T cell biology.
Maximize Scale
on Your Terms
Match the number of cells and samples to your study needs, with the flexibility to explore donors, conditions, and timepoints.
Connect Repertoire
to Cell State
Link paired alpha-beta TCR CDR3 sequences with whole transcriptome gene expression to characterize clonotypes and their associated T cell states.
Resolve Rare
Clonotypes
Identify low-frequency clonotypes with sensitive TCR detection to better characterize repertoire diversity and clonal expansion.
Maximize Flexibility
with Fixation
Collect and store samples across timepoints or locations, then process them together using an instrument-free workflow.
Configure Evercode
TCR MAX Around Your Study
Find the Evercode MAX TCR options that fit your sample count and target cell numbers, whether you’re studying individual donors or comparing larger cohorts.
Size Your Next Single Cell Experiment.
We’ll give you an instant quote on how much it may cost to run your experiment.
Connect Receptor Identity With T Cell State
Go beyond receptor sequences to understand the cells behind them. Pair TCR sequencing with whole transcriptome gene expression to identify T cell populations, characterize their states, and place clonotypes in their cellular context.
TCR Profiling Across
One Million Mouse T Cells
Nearly 1 million mouse T cells were profiled with Evercode TCR Mega. Detected TCRs mapped to annotated T cell populations, while an NKT-specific alpha chain localized to the NKT cluster, demonstrating how receptor identity can be connected to cell type.
High-Confidence TCR Pairing
Recover paired alpha-beta TCR CDR3 sequences to characterize clonotypes and investigate T cell responses. Evercode TCR demonstrates high pairing rates across antigen-stimulated T cells, providing a more complete view of the TCR repertoire.
High Paired Chain Recovery
Across Stimulated T Cells
Evercode TCR achieved paired alpha-beta CDR3 recovery in 94% of autoantigen-stimulated T cells and 85% of viral antigen-stimulated T cells, demonstrating high pairing rates across both populations.
Detect Rare T Cell Clonotypes
Evercode TCR has demonstrated detection of clonotypes at frequencies as low as 0.1% in controlled spike-in experiments. This sensitivity helps researchers characterize repertoire diversity and identify low-frequency clonotypes within larger T cell populations.
The Evercode™ TCR Workflow
The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.
Fixation
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Barcoding & Library Prep
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
Sequencing
The resulting libraries are sequenced by NGS.
Data Analysis
Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.
Frequently Asked Questions
What Is Single Cell TCR Sequencing?
What Does Evercode MAX TCR Measure?
Why Is Paired Alpha-Beta TCR Sequencing Important?
What Samples Can I Use with Evercode MAX TCR?
What Sequencing Depth Is Recommended for Evercode MAX TCR?
Does Evercode MAX TCR Require Specialized Instrumentation?
Can Evercode MAX TCR Detect Rare T Cell Clonotypes?
How Much Does Evercode MAX TCR Cost?
Resources and Support
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Read more about the technology and dive deeper into the technical details.
