Comparison of Evercode™ WT v4 and Chromium™ GEM-X Single Cell 3’ Kit v4 in Human PBMCs

Key Tech Note Takeaways

• Sensitivity comparison shows a marked increase in transcript detection in Evercode WT.
• Cell type proportions are equivalently represented.
• Reduced ambient RNA is observed with Evercode WT.
• Evercode exhibits significant decrease in mitochondrial and ribosomal reads.

Experimental Design

Frozen PBMCs from two donors (one female and one male) were obtained from a third-party provider. Aliquots derived from each donor were distributed to separate laboratories for processing. One aliquot per donor was shipped to a 10x Genomics Certified Service Provider for cell preparation and library construction using the Chromium GEM-X Single Cell 3’ Kit v4. Parallel aliquots were processed by Parse Biosciences using the Evercode WT v4 workflow. Sequencing libraries from both technologies were sequenced on the same sequencer model, and data were analyzed using each manufacturer’s respective analysis pipeline.