Evercode™ Whole Transcriptome v4 builds on the proven Evercode chemistry that researchers rely on, delivering higher sensitivity with less sequencing, and simpler workflow design for confident scaling of large, complex studies.
From the beginning, Evercode™ was designed to make scalable, instrument-free single cell transcriptomics accessible to every lab. Evercode WT v4 continues that evolution.
Evercode™ Whole Transcriptome v4 enhances gene and transcript detection across sequencing depths, delivering greater biological resolution while maintaining the flexibility and accessibility of combinatorial barcoding. No specialized instrument. No change to the underlying platform. Just refined performance built on the chemistry researchers already trust.
With higher transcript detection efficiency, v4 gives researchers flexibility in how sequencing is deployed. At standard sequencing depths, this translates into sharper resolution of rare cell states and lowly expressed genes. Alternatively, researchers can maintain a global transcriptomic view at lower read depths and redirect sequencing toward additional samples, conditions, or replicates.

Evercode™ Whole Transcriptome v4 introduces a magnetic bead-based workflow that enhances cell recovery and streamlines execution. By replacing centrifugation with bead-based clean-up, v4 delivers up to 75% higher cell retention.
The refined workflow reduces pipetting, supports automation, and increases confidence at critical handling steps, enabling researchers to maximize data from precious samples and scale studies with consistency.
Review side-by-side analyses of Evercode™ Whole Transcriptome v4 alongside Chromium GEM-X 3′ and Flex data.
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