Evercode™ WT Mini v3
SINGLE CELL WHOLE TRANSCRIPTOME

Single cell without the instrument

The Evercode™ WT Mini is perfectly sized to begin exploring single cell transcriptomics and experience the quality of results possible with Parse. Generate proof-of-concept results before you scale up to the Evercode™ WT or Evercode™ WT Mega to delve deeper into your biology.

See example datasets

10K

Cells

12

Samples

Evercode™ WT Mini

Up to 10K cells, 1-12 samples

Evercode™ WT

10K-100K cells, 1-48 samples

Evercode™ WT Mega

100K-1M cells, 1-384 samples

Evercode™ WT Penta

1M-5M cells, 1-384 samples

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

Get started with single cell

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

Exponentially scalable

Evercode's combinatorial barcoding enables you to dramatically scale up the cells and samples per experiment.

No instrument required

If you have a centrifuge, thermal cycler, and some pipettes, you’re ready to go.

Unmatched data quality

Better detect lowly expressed genes and avoid ambient RNA common in droplet-based single cell sequencing.

Works with fixed cells and nuclei

Fix and store samples as they come in for up to 6 months and then run together later on your schedule. Ideal for time-courses and cross-site collaborations.

Learn more about the technology

Higher sensitivity with Evercode

Evercode WT v2 detected an average of 84% more genes than the Chromium Next GEM Single Cell 3’ Kit v3.1 at the common read depth target of 20,000 reads/cell in mouse brain.

See the 10x comparison study

Start small.
Dream big.

The Evercode WT Mini Kit is your gateway to the world of single-cell transcriptomics. Lay the groundwork for comprehensive studies before scaling to the Evercode WT and Evercode WT Mega kits.

See the kit comparison study

Consistent quality at every scale

From the first experiment to large-scale projects, Evercode Whole Transcriptome ensures unwavering data quality. Begin your journey with peace of mind as data quality is unchanged as you scale.

Equivalent Cell Type Proportions

Relative abundance of each cell type for the available Evercode Whole Transcriptome v3 kits in identical human PBMC samples.

Lower multiplet rates than droplet-based approaches

In a human-mouse species mixing experiment using the WT, we detected only 2.3% multiplets—much lower than droplet-based approaches.

We detected rare cell types with Evercode’s higher sensitivity

Yi Xie

Duke-NUS Medical School

Enrico Petretto

Duke-NUS Medical School

Jacques Behmoaras

Duke-NUS Medical School

See 10x comparison study

The Parse Biosciences workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.

Fixation

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

Sequencing

The resulting libraries are sequenced by NGS.

Data Analysis

Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.

WT Mini: Resources and support

Get the Brochure

Read more about the technology and dive deeper into the technical details.

Download the brochure

Explore Related Data and Literature

Browse All Datasets

WT Mini
WT
+2

Comparison of Evercode™ Whole Transcriptome v3 kits in Human Immune Cells (PBMCs)

More Details

WT Mini
PBMCs

Performance of Evercode™ WT Mini v3 in Human PBMCs

More Details

WT Mini
Heart

Performance of Evercode™ WT in Neonatal Mouse Heart Cells

More Details

Evercode™ Whole Transcriptome Technical Brochure

More Details

Support

More information and literature across the entire range of Parse Biosciences products.

Support Suite

We're your partners in single cell

Reach out for a quote or for help planning your next experiment.

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ WT Penta

Resources

Trailmaker

Company

The Latest

Parse-Enabled Study Maps Neuronal Pathways with Potential Implications for ...

Vanderbilt Selects Parse Biosciences GigaLab to Generate Atlas of Early Neu...

Technology

The end-to-end solution

Products

Whole Transcriptome

Immune Profiling

Additional Capabilities

Evercode™ WT Penta

Resources

Trailmaker

Company

The Latest

Parse-Enabled Study Maps Neuronal Pathways with Potential Implications for ...

Vanderbilt Selects Parse Biosciences GigaLab to Generate Atlas of Early Neu...

Single cell without the instrument

10K

12

Evercode™ WT Mini

Evercode™ WT

Evercode™ WT Mega

Evercode™ WT Penta

Evercode™ WT Mini

Evercode™ WT

Evercode™ WT Mega

Evercode™ WT Penta

Get started with single cell

Exponentially scalable

No instrument required

Unmatched data quality

Works with fixed cells and nuclei

Higher sensitivity with Evercode

Start small.Dream big.

Consistent quality at every scale

Equivalent Cell Type Proportions

Lower multiplet rates than droplet-based approaches

We detected rare cell types with Evercode’s higher sensitivity

Yi Xie

Enrico Petretto

Jacques Behmoaras

The Parse Biosciences workflow

Fixation

Barcoding & Library Prep

Sequencing

Data Analysis

WT Mini: Resources and support

Get the Brochure

Explore Related Data and Literature

Comparison of Evercode™ Whole Transcriptome v3 kits in Human Immune Cells (PBMCs)

Performance of Evercode™ WT Mini v3 in Human PBMCs

Performance of Evercode™ WT in Neonatal Mouse Heart Cells

Evercode™ Whole Transcriptome Technical Brochure

Support

We're your partners in single cell

Technology

Products

Resources

Company

Start small.
Dream big.