Performance of Evercode™ WT in Mouse Lymph Node Nuclei

Fresh lymph node tissue, two inguinal and two brachial nodes, were collected from an adult CD-1 mouse and immediately processed. After isolation with the Singulator™ 100 (S2 Genomics), nuclei were strained, centrifuged, and resuspended in S2 Genomics Nuclei Storage Buffer and RNase Inhibitor. The single nuclei suspension was fixed with Evercode Nuclei Fixation v2, and then whole transcriptome libraries were created with Evercode WT v2.

One whole transcriptome sublibrary was sequenced on an Illumina© Nextseq™ 550 High Output Kit v2.5 (150 Cycles), and data was processed with Parse Biosciences Analysis Pipeline v1.0.5. The resulting 12,550 cells were clustered with Seurat v4.0, manually annotated, and visualized as UMAPs. Our analysis revealed the expected cell types, as shown in the UMAP below.

Next Steps

• Explore the data and do your own analysis by downloading the raw data below.
• These samples were collected and processed in parallel with 10x Chromium Next GEM Single Cell 3’ Kit v3.1. Explore how this data compares to that technology in Evercode™ WT v2 vs. Chromium™ Next GEM Single Cell 3’ Kit v3.1 in Mouse Lymph Node Nuclei Tech Note
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