Superb-seq Uncovers Hidden Off-Target Edits with CRISPR + Single-Cell Precision
Key Takeaways:
- Superb-seq enables simultaneous detection of on- and off-target CRISPR edits at single cell resolution by combining T7 in situ transcription with Evercode™ scRNA-seq.
- Sheriff software quantifies edit sites and transcriptomic effects, revealing 36 off-target sites alongside 7 on-target edits across 7 guide RNAs.
- Off-target edits can disrupt gene expression through non-coding regions, as shown by USP9X regulation loss due to a CHD3 guide-induced off-target edit.
This dataset is licensed under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) license. Non-commercial users are free to share and adapt the data by providing appropriate credit – Parse Biosciences citation guidelines are available here. If you’re interested in licensing the data for commercial purposes, visit our Dataset Commercial Licensing page.
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