Evercode™ TCR Single Cell Immune Profiling at Scale Connect with a single cell expert TCR Mini Explore the benefits of single cell TCR sequencing. 10k Cells 12 Samples TCR Comprehensive TCR sequencing across samples, replicates, or timepoints. 100k Cells 48 Samples TCR Mega Capture the diversity of the immune repertoire at scale. 1M Cells 96 Samples T CELL RECEPTOR + WHOLE TRANSCRIPTOMEIMMUNE PROFILING WITHOUT THE INSTRUMENT T Cell Receptor (TCR) Profiling T cells play a central role in the adaptive immune system. The T Cell Receptor (TCR) is a protein complex that enables recognition and response to an enormous breadth of antigens. Each TCR is composed of two chains that combine and add to the diversity of a possible receptor. In most cases, these consist of the alpha chain (TCR alpha) and the beta chain (TCR beta). The enormous diversity of the TCR results from recombination of the variable (V), the diversity (D), and the joining (J) gene segments and the insertion of random nucleotides at junctions. Gene Expression clustering of T cells Gene expression on nearly 1 million T cells isolated from PBMCs. In addition to capturing their repertoire, cells can be classified into T cell subtypes. Up to 1 Million Cells in a Single Experiment Scale matters. The diverse immune repertoire demands higher cell number experiments to identify the vast numbers of TCR clonotypes present in each sample. Sensitive Clonotype Detection Revealing the complexity of the immune repertoire requires sensitive detection of TCRs along with whole transcriptome profiles. TCR chain assignment and pairing High chain detection and pairing percentage of alpha and beta chains in both activated T cells and primary T cells. Number of unique beta chain clonotypes across donors Detect large numbers of low frequency, rare clonotypes (1 or 2 cells) among expanded clones. Comprehensive Immune Repertoire Detection The Evercode TCR kit captures the largest single cell immune repertoire dataset from a single experiment. THE PARSE BIOSCIENCES WORKFLOW From Bench to Insight The Evercode TCR and Whole Transcriptome technology provide the reagents, software, and accessibility to pursue difficult research questions. 1 Fixation Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding. 2 Barcoding & Library Prep Append barcodes to each transcript by progressing cells through four split-pool combinatorial barcoding steps. The kit proceeds to library preparation steps to generate both TCR and whole transcriptome sequencing ready libraries. 3 Sequencing The resulting libraries are sequenced by NGS. 4 Data Analysis Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy. Get the Brochure Read more about the technology with a deeper dive into the technical details. Download the Brochure Support Information and insight for the entire range of Parse Biosciences products. Support Suite Advancing Research for 850+ Labs
