Our kits take you from single cell or single nuclei suspensions through sequencing straight into biological insights.
Target 100s to 1000s of genes to analyze more samples with less sequencing.
Pair guide RNAs with single cell whole transcriptomes.
Transform single cell sequencing output into understandable results.
Parse Biosciences provides researchers with the ability to perform single cell sequencing with unprecedented scale and ease.
A Seattle-based company with the mission of accelerating progress in human health and scientific research.
Customer interviews, single cell sequencing tips and tricks, and the latest updates from Parse Biosciences.
A list of upcoming conferences and webinars we are attending.
The latest news stories and press releases from Parse Biosciences.
Explore our library of resources to learn more about Evercode technology and some applications from leading researchers.
Download publications and posters featuring the Evercode technology in single cell research.
Explore datasets covering various applications and sample types or access product details.
Upcoming & past webinars on Evercode technology and its applications.
Information and insight for the entire range of Parse Biosciences products.
Fill out this form to get your questions answered by Parse Biosciences single cell experts.
Evercode TCR recovered paired TCR alpha and TCR beta chains in 82% of activated human T cells, along with their whole transcriptome.
Isolated T cells from human healthy donor PBMCs were placed in culture for 3 days and activated with CD3/CD28 beads and IL-2. Evercode Cell Fixation Kit v2 was used to stabilize the cell structure and protect the RNA transcriptome from degradation and fixed cells were stored at -80C prior to barcoding and library prep. The Evercode TCR kit generated whole transcriptome and TCR specific libraries that were sequenced on an Illumina Nextseq 550 using High Output and Mid Output Kits v2.5, respectively. The resulting data for 80,000 cells was processed with Parse Biosciences Analysis Pipeline v1.0.4.
Talk to our team to request a quote or plan your next experiment.
Detection of individual TCR alpha chains (90% of cells) and beta chains (92% of cells) in activated T cells demonstrate high sensitivity of the assay. Median transcripts per cell were 2,016 while median genes per cell were 1,480 at a sequencing depth of 5,000 reads/cell.
A set of primary human T cells were assayed without activation for comparison. TCR detection rates from both the activated and primary T cells are shown below. The primary T cell dataset can be explored in detail here.
Experimental Summary Report (HTML)
TCR Results Clonotype Frequency (TSV) Barcode Report (TSV) The Adaptive Immune Receptor Repertoire (AIRR) File (TSV)
Whole Transcriptome Results Digital Gene Expression (DGE) Matrix (1.5 GB) All Gene (CSV) Cell Metadata (CSV)
Primary T cells dataset