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Datasets

Performance of Evercode™ WT in Mouse Kidney Nuclei

WT v2

Fresh kidney tissue was collected from an adult CD-1 mouse and immediately processed. After isolation with the Singulator 100 (S2 Genomics™), nuclei were strained, centrifuged, and resuspended in Nuclei Storage Buffer. The single nuclei suspension was fixed with Evercode Nuclei Fixation v2 then whole transcriptome libraries were created with Evercode WT v2. 

Libraries were sequenced on an Illumina Novaseq 6000, and data was processed with Parse Biosciences Analysis Pipeline v1.0.2. The 19,779 nuclei were clustered with Seurat v4.0, manually annotated, and visualized as UMAPs. Our analysis revealed the expected cell types, as shown in the UMAP below. 

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You can further explore the data and do your own analysis by downloading the raw data below.

Downloads

Experimental Summary Reports, Digital Gene Expression (DGE) Matrix, All Gene, and Cell Metadata for Evercode WT v2.

Experimental Summary Report (HTML)
Digital Gene Expression (DGE) Matrix (659 MB)
All Gene (CSV)
Cell Metadata (CSV)