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Harness the Power of Evercode™ Combinatorial Barcoding

Talk to a Single Cell Specialist
Break the bounds of droplet technology

The Cell is the
Reaction Vessel

The Evercode™ solution converts each cell or nucleus into an individualized reaction compartment. This approach steps away from the limitations of hardware-based offerings by evolving the assay for a more elegant, simple solution to single cell (scRNA-Seq) and single nuclei sequencing (snRNA-Seq).

Combinatorial barcoding happens within cells themselves – resulting in extraordinary specificity and scalability.

The Next Evolution of Single Cell RNA-Seq

Fix Now, Run Later

Fix cells or nuclei as they're available to lock in the biology. After a 30 minute fixation, samples are stable for 6 months. Whether working on a timecourse study, sharing samples between laboratories, or segregating sample preparation from core lab space, fixation provides the flexibility to work the way you desire.
Cells and Nuclei on Your Schedule

No Instrument Required

Conventional droplet-based single-cell technologies struggle as cell or experiment sizes change. Parse makes it easy to scale your experiments regardless of cell size or sample type. The Evercode™ Whole Transcriptome technology, originally based on the split-pool combinatorial barcoding method published in Science and known widely as SPLiT-Seq, is accessible to any standard biology lab.

1

Reverse Transcription

Split | Samples are distributed into wells and the first, sample-specific barcodes are applied to fixed cells or nuclei with an in-cell reverse transcription (RT) reaction.

Reverse Transcription
2

Pool | Cells from each well are pooled together.

3

Ligation

Split | Cells or nuclei are distributed across a plate and an in-cell ligation appends the second barcode.

Ligation
4

Pool | Cells from each well are pooled together.

5

Ligation

Split | A third barcode is applied with another in-cell ligation after the cells or nuclei are split across a plate.

Ligation
6

Pool | Cells from each well are pooled together.

7

Lysis + PCR

Split | The pooled cells are divided across several sublibraries. The cells are lysed and the fourth, sublibrary-specific barcode is applied by PCR.

Lysis + PCR
8

Library Prep

Split | The library preparation appends adapters ready for loading on any Illumina sequencer.

Library Prep

Unmatched Sensitivity at Any Scale

Run more samples, use less sequencing, and delve deeper into the biology with gene and transcript detection that outperforms droplet-based methods. Evercode™ combinatorial barcoding technology works inside individual cells in a highly parallel fashion, resulting in unmatched data quality regardless of experimental size.

Better Gene Detection Than Droplet-Based Approaches
We've tested Evercode™ combinatorial barcoding technology in multiple cell types and always outperform the leading droplet-based technology - up to half the sequencing required for the same number of detected genes (Evercode™ WT v1 results shown).
Boundless Scale Boundless Scale

Insights Included

Our data analysis package transforms sequencing output into understandable results, enabling the ability to assess data quality, identify sample differences, and interrogate genes of interest. Each analysis provides an interactive web report allowing for anyone to easily browse the data — in addition to more detailed files that allow for more complex downstream analysis.

Parse Software

Compatible with any NGS Sequencer

Starting from FASTQ, the included data analysis pipeline performs several data quality checks and sequencing error corrections. A rigorous computational biology approach maps results back to individual cells, performs cell clustering, and calculates differential expression to inform experimental insights.

Seamless Integration with 3rd Party Tools

Many biological experiments require specialized bioinformatic analysis or integration with an existing toolset for compatibility and comparison with previous results. We provide tutorials in our support area for integrating the output gene count matrix files with third-party tools to facilitate research in your platform of preference.

Interactive Results

The data analysis pipeline translates the sequencing outputs into a report for experiment QC or deep biological exploration. We’ve worked with leaders in the field to present the most informative summary statistics: cell coverage and sequencing depth, a cell cutoff graph, and sequencing saturation curves. When it’s time to dive deeper, differential expression results can be mined alongside an interactive UMAP for rapid investigation of genes of interest.