Gene Capture Targeted Analysis for Greater Scalability Connect with a single cell expert Custom Gene Capture Design a panel tailored to each project. Immune1000 Gene Capture Target human immune cell type markers and pathways. Targeted Panels for Single Cell RNA-seq Unlock Greater Scalability Many research applications are focused on a discrete set of genes and do not require whole transcriptome analysis in every experiment. Gene Capture enables researchers to answer questions across more samples with less sequencing – expanding the utility of the Evercode product line. Gene Capture Preserves Cell Type Clustering Human PBMCs processed and sequenced with Evercode WT v2 at 28k reads/cell (left) and Immune1000 Gene Capture at 2k reads/cell (right). The resolution and identification of cell types is preserved at lower sequencing. High Resolution Data With 10 Times Less Sequencing With a high on-target rate, shallow sequencing maintains excellent resolution and cell type proportions. Maintain Cell Type Proportions The Immune1000 Gene Capture is optimized to avoid bias during enrichment, preserving cell type proportions for the sample. Cell type proportions are retained Human PBMCs processed with Evercode WT v2 and Immune1000 Gene Capture show excellent concordance for cell type proportionality. Design a Custom Panel of Target Genes Gene Capture can be tailored to individual research goals, targeting 100s or 1000s of genes of interest. THE GENE CAPTURE WORKFLOW Complete Workflow Gene Capture is an add-on to Evercode Whole Transcriptome. Whole Transcriptome libraries are hybridized to panels of gene-specific probes. Library molecules containing target regions are captured with magnetic beads. 1 Evercode Cell/Nuclei Fixation Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding. 2 Evercode Whole Transcriptome Append barcodes to each transcript by progressing cells through four split-pool combinatorial barcoding steps, then proceed for library preparation. 3 Gene Capture Targeted transcripts are hybridized to a designed probe and captured with magnetic beads. 4 Sequencing The targeted libraries are sequenced by NGS. 5 Analysis A computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy. Learn More Read more about the technology and Immune1000 Gene Panel. Download Product Sheet Download Immune1000 Panel Gene List Explore the Sample Data Investigate the comparison between PBMCs prepared with and without Immune1000 Gene Capture. Explore sample data Whole Transcriptome in Up to 1 Million Cells Sequence up to 1 million cells, no instrument required. Evercode™ WT Mega Support Information and insight for the entire range of Parse Biosciences products. Support Suite Advancing Research for 850+ Labs
