CRISPR Detect Single Cell CRISPR Screening at Unprecedented Scale Connect with a single cell expert PAIRED GUIDE RNA &GENE EXPRESSION A New Era of CRISPR Screening Single cell pooled CRISPR screens enable analysis of complex phenotypes by linking individual gene perturbations with gene expression profiles. This approach is used for target validation in drug discovery, to understand differences in treatment responses, and map pathways involved in cell differentiation. Wider adoption of this approach is constrained by the feasibility of processing sufficient cells for libraries containing more than a few hundred guide RNAs (sgRNAs). CRISPR Detect brings the scalability of Evercode technology to single cell CRISPR screens, making genome-wide studies practical. Number of Runs in Recently Published CRISPR Screens The published number of 10x Genomics Chromium Next GEM Single 3’ Kit lanes and projected Evercode Whole Transcriptome kits to obtain the same number of cells. More Perturbations in Fewer Runs Scale easily with CRISPR Detect and Evercode Whole Transcriptome kits. Sensitive Guide Detection Avoid underpowered analysis and wasted sequencing data. High Guide RNA Detection with Low Background LN18 cells were prepared so they can only express one sgRNA then analyzed with Evercode WT Mega v2 and CRISPR Detect. THE CRISPR DETECT WORKFLOW Complete Workflow CRISPR Detect is a powerful addition to Evercode Whole Transcriptome capabilities, and seamlessly integrates into the same workflow. 1 Evercode Cell Fixation Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding. 2 Evercode Whole Transcriptome Append barcodes to each transcript by progressing cells through split-pool combinatorial barcoding, then proceed for library preparation. 3 CRISPR Detect CRISPR Detect pairs with Evercode Whole Transcriptome to enrich sgRNA sequences. 4 Sequencing Evercode Whole Transcriptome and CRISPR Detect libraries are sequenced together. 5 Analysis Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix and sgRNA assignments, integrate seamlessly with existing open source tools such as Seurat or Scanpy. Get the Product Sheet Read more about the technology with a deeper dive into the technical details. Download Product Sheet Download Vector Compatibility Guide Explore the Sample Data Investigate paired sgRNA CRISPR Detect and Evercode WT Mega data. Explore sample data Whole Transcriptome in Up to 1 Million Cells Sequence up to 1 million cells, no instrument required. Evercode™ WT Mega Support Information and insight for the entire range of Parse Biosciences products. Support Suite Advancing Research for 850+ Labs
