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We explored the feasibility of using the Evercode combinatorial barcoding kit to perform short-read Illumina sequencing and Oxford nanopore long-read sequencing from a split library consisting of human induced pluripotent stem cells and a AC16 cardiac cell line. Our goals are to better understand how transcriptional variants are realized into a diversity of protein and cellular phenotypes and the genetic structures that give rise to meaningful transcriptional variants.
The data presented here is from our pilot experiment and the development of modifications to handle ONT long-reads in the Evercode pipeline. Next steps are scaling up the experiment for the WT kit, increasing the sequencing depth and cell retention.
Illumina Short Read Experimental Summary Report (HTML) Digital Gene Expression (DGE) Matrix (127 MB) All Gene (CSV) Cell Metadata (CSV)
ONT Long Read Experimental Summary Report (HTML) Digital Gene Expression (DGE) Matrix (5 MB) All Gene (CSV) Cell Metadata (CSV)