Evercode™ Whole Transcriptome products are enabling scientists to push scRNA-seq past the limitations of previous technologies to scale up the samples and cells per experiment while also detecting more genes per cell. And all with no instrument purchase required.
Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab
Evercode コンビナトリアル・
バーコード・テクノロジーにより
1回の実験で処理できる細胞数と
サンプル数を大幅に拡大可能。
遠心機、サーマルサイクラー、
ピペットがあれば、すぐに実験を
開始可能。
低発現遺伝子をより正確に検出し、
ドロップレット法に伴う環境RNAの
混入を回避。
固定したサンプルを最大6か月間、
保存でき、スケジュールに応じて
まとめて解析が可能。タイムコース
実験や拠点間の共同研究に最適。
Merge past and present research efforts, maintaining continuity in your data while exploring new frontiers.
Single cell data from 17k mouse brain nuclei analyzed by Evercode WT v2 and Chromium Next GEM Single Cell 3' v3.1 were integrated, clustered, and annotated - identifying the same cell types.
Parse Biosciences Evercode™ WT v2 combinatorial barcoding technology was compared with the droplet-based 10x Genomics™ Chromium™ Next GEM Single Cell 3’ Kit v3.1 with mouse brain nuclei. This heterogeneous sample type has been the focus of an array of cell atlas projects and individual researchers.
View comparison results
Two E18 embryonic C57/BI6 samples were collected, sagittally dissected into 2 halves, and flash frozen by a third-party tissue vendor. Half of each brain was shipped to a 10x Genomics™ Certified Service Provider, and they isolated nuclei with the Chromium™ Nuclei Isolation Kit and created sequencing libraries with the Chromium™ Next GEM Single Cell 3’ GEM Library & Gel Bead Kit v3.1. The other halves of each brain were processed by Parse Biosciences for nuclei isolation with a dounce homogenizer, fixation with Evercode™ Nuclei Fixation v2, and library preparation with Evercode™ WT v2. Sequencing libraries from each technology were sequenced by a third party. The sequencing data were analyzed with each manufacturer’s data analysis pipeline.
Multiplets are a nuisance of scRNA-seq approaches that complicate data analysis. When the cell is the reaction vessel, issues related to multiple cells in a droplet are eliminated, thus improving data cleanliness.
In a human-mouse species mixing experiment using the WT, we detected only 2.3% multiplets—much lower than droplet-based approaches.
The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
The resulting libraries are sequenced by NGS.
Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.
Read more about the technology and dive deeper into the technical details.
More information and literature across the entire range of Parse Biosciences products.
Reach out for a quote or for help planning your next experiment.
