Technology

Discover scalable, instrument-free single cell sequencing technology from Parse Bioscience

Technology Overview


リソース

Explore our collection of resources to learn more about technology and its applications from leading researchers

Resources Overview

会社情報

研究者に前例のない規模と容易さでシングルセルシーケンスを提供

Parseについて

Evercode™ WT Mini v3
SINGLE CELL WHOLE TRANSCRIPTOME

Single cell without the instrument

The Evercode™ WT Mini is perfectly sized to begin exploring single cell transcriptomics and experience the quality of results possible with Parse. Generate proof-of-concept results before you scale up to the Evercode™ WT or Evercode™ WT Mega to delve deeper into your biology.

10K

Cells

12

Samples

EVERCODE™ TECHNOLOGY MAKES IT ALL POSSIBLE

シングルセル解析を始めましょう

Evercode™ split-pool combinatorial barcoding is a simple, instrument-free solution to single cell sequencing. This easily adopted approach brings unprecedented sensitivity, scalability, and flexibility to any lab.

飛躍的な
スケーラビリティ

Evercode コンビナトリアル・
バーコード・テクノロジーにより
1回の実験で処理できる細胞数と
サンプル数を大幅に拡大可能。

専用機器は不要

遠心機、サーマルサイクラー、
ピペットがあれば、すぐに実験を
開始可能。

比類のない
データ品質

低発現遺伝子をより正確に検出し、
ドロップレット法に伴う環境RNAの
混入を回避。

Works with fixed cells and nuclei

固定したサンプルを最大6か月間、
保存でき、スケジュールに応じて
まとめて解析が可能。タイムコース
実験や拠点間の共同研究に最適。

Learn more about the technology

Higher sensitivity with Evercode

Evercode WT v2 は、マウス脳サンプルにおいて、Chromium Next GEM Single Cell 3’
Kit v3.1 と比較し、共通のリードデプス(20,000リード/細胞)で平均84%多くの
遺伝子を検出した。

10x との比較研究を見る

Start small.
Dream big.

The Evercode WT Mini Kit is your gateway to the world of single-cell transcriptomics. Lay the groundwork for comprehensive studies before scaling to the Evercode WT and Evercode WT Mega kits.

See the kit comparison study

Consistent quality at every scale

From the first experiment to large-scale projects, Evercode Whole Transcriptome ensures unwavering data quality. Begin your journey with peace of mind as data quality is unchanged as you scale.

Equivalent Cell Type Proportions

Relative abundance of each cell type for the available Evercode Whole Transcriptome v3 kits in identical human PBMC samples.

Lower multiplet rates than droplet-based approaches

In a human-mouse species mixing experiment using the WT, we detected
only 2.3% multiplets—much lower than droplet-based approaches.

Evercode の高感度により希少な細胞種を検出


Yi Xie

Duke-NUS メディカルスクール

Enrico Petretto

Duke-NUS メディカルスクール

Jacques Behmoaras

Duke-NUS メディカルスクール

The Parse Biosciences workflow

The Evercode™ Whole Transcriptome solution provides the reagents, software, and support to pursue difficult research questions from bench to insight.

1

固定

Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.

2

Barcoding & Library Prep

Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.

3

シーケンス

The resulting libraries are sequenced by NGS.

4

データ解析

Our Trailmaker platform provides an end-to-end data analysis experience, guiding you from FASTQ files to publication-ready figures, while maintaining the flexibility of multiple data entry and exit points. Our computational pipeline continues to also be available.

WT Mini: Resources and support

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Read more about the technology and dive deeper into the technical details.

Support

More information and literature across the entire range of Parse Biosciences products.

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