Explore a wide variety of applications across the adaptive immune system through Evercode™ TCR. Without needing to purchase proprietary hardware, your lab can delve into the immune response, detecting paired alpha-beta chains to characterize the immune repertoire complexity.
Up to 10K cells, 1-12 samples
10K-100K cells, 1-48 samples
100K-1M cells, 1-96 samples
Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.
Capture the diverse immune repertoire with the power of 1 million T cells. The Evercode TCR Mega kit enables the study of TCR clonotype diversity and T cell states at an unprecedented resolution.
Understanding the immune repertoire diversity necessitates sensitive detection of TCRs along with whole transcriptome profiles.
In an experiment profiling viral or autoantigen stimulated T cells, Evercode TCR captured paired alpha and beta chains in 85-94% of cells.
Over half a million unique beta chain clonotypes were identified across 7 timepoints in mouse lifespan, showing a clear decrease in clonotype diversity with age.
Parse makes it easy to batch samples with fixation. With Evercode TCR, fix and store samples as they come in and then run the samples together later on your schedule.
The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
The resulting libraries are sequenced by NGS.
Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology and dive deeper into the technical details.
More information and literature across the entire range of Parse Biosciences products.
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