製品
The Evercode™ TCR Mini is perfect to start your single cell immune repertoire sequencing project. Generate single cell TCR and gene expression data without needing to purchase an expensive droplet-based instrument.
Cells
Samples
Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.
Isolated T cells from peripheral blood mononuclear cells (PBMCs) were either directly profiled (Primary) or placed in culture for 3 days and activated with CD3/CD28 beads and IL-2 (Activated).
The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
The resulting libraries are sequenced by NGS.
Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology and dive deeper into the technical details.
More information and literature across the entire range of Parse Biosciences products.
Reach out for a quote or for help planning your next experiment.