製品
When dealing with a system as complex as the full repertoire of immunity, more is better. Achieve unpresecented resolution as you study TCR clonotype diversity and T-cell states. Evercode™ TCR Mega offers scale alongside a host of other benefits, with the added information provided by WT profiles.
Cells
Samples
Reveal the complexity of the immune repertoire with sensitive detection of TCRs. Measure transcriptome-wide gene expression (including key T-cell subtype markers) together with paired alpha and beta sequences in the same cells.
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Scale matters. Uncovering the vast diversity in the immune repertoire requires large cell experiments. Identify more TCR clonotypes than ever before with Evercode.
Gene expression on nearly 1 million T cells isolated from PBMCs. In addition to capturing their repertoire, cells can be classified into T cell subtypes.
Isolated T-cells from peripheral blood mononuclear cells (PBMCs) were either directly profiled (Primary) or placed in culture for 3 days and activated with CD3/CD28 beads and IL-2 (Activated).
The Evercode TCR kit captures the largest single cell immune repertoire dataset from a single experiment.
The Evercode™ TCR solution provides the reagents, software, and accessibility to pursue difficult research questions.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through a streamlined split-pool combinatorial barcoding process, which produces sequencing-ready libraries.
The resulting libraries are sequenced by NGS.
Our computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology and dive deeper into the technical details.
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