Evercode™ WT v3 and Chromium™ Single Cell Gene Expression Flex Kit in Fixed Mouse Lymph Node Nuclei

Key Tech Note Takeaways

• Head-to-head comparison shows increased gene body coverage
• Twice as many unique genes detected, including diverse novel RNA biotypes
• Probe-based methods produce inconsistent measurements in comparison to RT-based methods

Experimental Design

Mouse lymph node tissue was dissociated into a single nuclei solution with a Singulator™ 100/200 (S2 Genomics). The samples were strained, centrifuged, and resuspended in S2 Genomics Nuclei Storage Buffer and RNase Inhibitor. The sample was split, and half of the sample was fixed and prepared with the 10x Genomics Single Cell Flex Kit. The remaining half was fixed with Evercode Cell Fixation v3 and shipped for further processing with Evercode WT v3. The sequencing data were processed with each manufacturer’s respective analysis pipeline.

Explore the Data