Superb-seq Uncovers Hidden Off-Target Edits with CRISPR + Single-Cell Precision

Key Takeaways:

• Superb-seq enables simultaneous detection of on- and off-target CRISPR edits at single cell resolution by combining T7 in situ transcription with Evercode™ scRNA-seq.
• Sheriff software quantifies edit sites and transcriptomic effects, revealing 36 off-target sites alongside 7 on-target edits across 7 guide RNAs.
• Off-target edits can disrupt gene expression through non-coding regions, as shown by USP9X regulation loss due to a CHD3 guide-induced off-target edit.

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