Datasets

Performance of Evercode™ WT in Mouse Liver Nuclei

WT V1 V2
Adult Mouse Liver Nuclei
  • Explore the performance of the WT v2 and WT v1 products in nuclei
  • Review key areas of improvement in the updated product in areas such as robustness across samples and sensitivity (75% more genes)
  • Confirm the expression profiles are comparable and highly informative

Performance of Evercode™ WT in Mouse Brain Nuclei

WT V1 V2
E18 Mouse Brain from 2 Donors
  • Explore the performance of the WT v2 and WT v1 products in nuclei
  • Review key areas of improvement in the updated product in areas such as gene sensitivity (37% more genes)
  • Confirm the expression profiles are comparable and highly informative

Performance of Evercode™ WT in a Multi-Species Cell Line Experiment

WT V1 V2
Human HEK293 and Mouse NIH/3T3 Cell Lines
  • Explore the performance of the WT v2 and WT v1 products in cells
  • Compare the low multiplet rates across versions
  • Confirm the expression profiles are comparable and highly informative

Performance of Evercode™ WT in Human Immune Cells (PBMCs)

WT V1 V2
Human Peripheral Blood Mononuclear Cells from 4 Donors
  • Explore the performance of the WT v2 and WT v1 products in cells
  • Review key areas of improvement in the updated product in areas such as robustness across samples
  • Confirm the expression profiles are comparable and highly informative

High sensitivity single cell RNA sequencing with split pool barcoding

WT V1 V2

Abstract

Single cell RNA sequencing (scRNA-seq) has become a core tool for researchers to understand biology. As scRNA-seq has become more ubiquitous, many applications demand higher scalability and sensitivity. Split-pool combinatorial barcoding makes it possible to scale projects to hundreds of samples and millions of cells, overcoming limitations of previous droplet based technologies...

doi: https://doi.org/10.1101/2022.08.27.505512

Single-cell RNA-sequencing of 1 million Human cells in a single experiment

V1
24 PBMC samples total: 12 with Type-1 Diabetes and 12 healthy controls
  • Twenty-four samples were collected and fixed over three weeks, then processed in a single scRNA-seq experiment.
  • Over 27,000 cells were captured per sample.
  • Low frequency and fragile cell types like both types of classical dendritic cells (cDC1 and cDC2), and plasmacytoid dendritic cells (pDC) were identified in both healthy and diabetic samples.

Collection of Experiments from Evercode™ Whole Transcriptome v1 Product

WT V1
  • Explore the high sensitivity of the Whole Transcriptome Kit’s gene detection in a mix of human and mouse cell lines.
  • See how combinatorial barcoding enables high throughput sequencing of cells, with much lower doublet rates than droplet solutions.

Evercode™ Whole Transcriptome Technical Brochure

  • Learn how the Evercode split-pool combinatorial barcoding leads to improved experimental design and eliminates the need for special equipment.
  • Understand how Evercode improves data quality and achieves robust gene detection across different sample types.

Evercode™ Whole Transcriptome Product Sheet

  • More genes with less sequencing
  • More consistent gene detection
  • Unbiased gene expression