Publications Archive - Page 4 of 5

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Mouse Lung

Common human genetic variants of APOE impact murine COVID-19 mortality

Benjamin N. Ostendorf, Mira A. Patel, Jana Bilanovic, H.-Heinrich Hoffmann, Sebastian E. Carrasco, Charles M. Rice, and Sohail. F. Tavazoie

Lung tissue from knock-in mice expressing common variants of human APOE (APOE2, APOE3 and APO4) showed differences in gene expression that causally impacted the outcome of COVID-19 infection. APOE2 and APOE4 knock-in mice had increased disease progression and mortality relative to APOE3 knock-in mice. Human data from the UK Biobank supports the finding that genetic variation in APOE partially explains the variation in severity of COVID-19 disease outcomes.

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Ovarian PDX

A Risk-reward Examination of Sample Multiplexing Reagents for Single Cell RNA-Seq

Daniel V. Brown, Casey J.A. Anttila, Ling Ling, Patrick Grave, Tracey M. Baldwin, Ryan Munnings, Anthony J. Farchione, Vanessa L. Bryant, Amelia Dunstone, Christine Biben, Samir Taoudi, Tom S. Weber, Shalin H. Naik, Anthony Hadla, Holly E. Barker, Cassandra J. Vandenberg, Genevieve Dall, Clare L. Scott, Zachery Moore, James R. Whittle, Saskia Freytag, Sarah A. Best, Anthony T. Papenfuss, Sam W.Z. Olechnowicz, Sarah E. MacRaild, Stephen Wilcox, Peter F. Hickey, Daniela Amann-Zalcenstein, Rory Bowden

The authors compared various scRNA-Seq reagents. Parse WT v2 and 10x Flex were tested on fixed tumor nuclei. 10x Flex failed due to clogging. Parse data had more reads, genes, and fewer doublets than 10x fresh nuclei data. 10X data had high mitochondrial and ribosomal transcripts, but gene expression was concordant with Parse data. Parse WT v2 captured more biological variation and outperformed other assays.

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WT Mega
Human IPSCs

Directed Differentiation of Human iPSCs to Functional Ovarian Granulosa-Like Cells via Transcription Factor Overexpression

Merrick Pierson Smela, Christian Kramme, Patrick Fortuna, Jessica Adams, Edward Dong, Mutsumi Kobayashi, Garyk Brixi, Emma Tysinger, Richie. E. Kohman, Toshi Shioda, Pranam Chatterjee, and George M. Church

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Mouse Brain

Absence of microglia promotes diverse pathologies and early lethality in Alzheimer’s disease mice

Sepideh Kiani Shabestari, Samuel Morabito, Emma Pascal Danhash, Amanda McQuade, Jessica Ramirez Sanchez, Emily Miyoshi, Jean Paul Chadarevian, Christel Claes, Morgan Alexandra Coburn, Jonathan Hasselmann, Jorge Hidalgo, Kayla Nhi Tran, Alessandra C. Martini, Winston Chang Rothermich, Jesse Pascual, Elizabeth Head, David A. Hume, Clare Pridans, Hayk Davtyan, Vivek Swarup, and Mathew Blurton-Jones

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Cell Lines

Mapping and modeling the genomic basis of differential RNA isoform expression at single-cell resolution with LR-Split-seq

Elisabeth Rebboah, Fairlie Reese, Katherine Williams, Gabriela Balderrama-Gutierrez, Cassandra McGill, Diane Trout, Isaryhia Rodriguez, Heidi Liang, Barbara J. Wold, and Ali Mortazavi

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Whole Transcriptome

Immune Profiling

Additional Capabilities

Publications and Posters

Common human genetic variants of APOE impact murine COVID-19 mortality

A Risk-reward Examination of Sample Multiplexing Reagents for Single Cell RNA-Seq

Publications

Directed Differentiation of Human iPSCs to Functional Ovarian Granulosa-Like Cells via Transcription Factor Overexpression

Absence of microglia promotes diverse pathologies and early lethality in Alzheimer’s disease mice

Mapping and modeling the genomic basis of differential RNA isoform expression at single-cell resolution with LR-Split-seq

Posters

Automation of the Parse Biosciences Evercode Workflow with the INTEGRA Assist Plus Liquid Handler

Automated Single-Cell Sequencing Using Evercode Split-Pool Combinatorial Barcoding on a Hamilton Vantage Liquid Handler

Targeted Transcriptome Sequencing Enables Exponential Scaling of Combinatorial Barcoding

Evercode Whole Transcriptome Single-Cell RNA-Seq with Focal Barcoding Enables Single-Cell Transcriptomes Coupled with Gene or Vector-Enriched Readouts

Improving Sensitivity of Combinatorial Barcoding-based Single Cell Sequencing

Scalable & Sensitive Single Cell RNA-seq with Combinatorial Barcoding

Single Cell Sequencing Using the Parse Biosciences Evercode™ WT Kit on the G4™ Platform

Fast and gentle multiparametric cell sorting upstream of transcriptomics via split-pool combinatorial barcoding

Isolation of neural cell subsets using a fast and gentle microfluidic cell sorter results in highly viable cells that can be used for downstream culture, imaging, and transcriptomics