Design a panel tailored to each project.
Target human immune cell type markers and pathways.
You have cells of interest. We have an add-on solution that delivers expanded throughput while saving on sequencing costs. Choose the Immune1000 panel of genes, or design a Custom Gene Capture panel of your own. When you’re ready, we’ll help you plan.
Design a panel tailored to each project.
Target human immune cell type markers and pathways.
Many research applications are focused on a discrete set of genes and do not require whole transcriptome analysis in every experiment. Gene Capture selects only the genes of interest, enabling researchers to answer questions across more samples with less sequencing.
With a high on-target rate, shallow sequencing maintains excellent resolution and cell type proportions.
Human PBMCs processed and sequenced with Evercode™ WT v2 at 28K reads/cell (left) and Immune1000 Gene Capture at 2K reads/cell (right). The resolution and identification of cell types is preserved at lower sequencing.
Human PBMCs processed with Evercode™ WT v2 and Immune1000 Gene Capture show excellent concordance between cell type proportions.
The Immune1000 Gene Capture preserves cell type proportions within samples.
Gene Capture is an add-on to Evercode™ WT. WT libraries are hybridized to panels of gene-specific probes. Library molecules containing target regions are captured with magnetic beads.
Lock in gene expression immediately after sample collection with a rapid fixation protocol. After fixation, samples can be stored for up to 6 months or proceed directly to barcoding.
Append barcodes to each transcript by progressing cells through 4 split-pool combinatorial barcoding steps, then proceed for library preparation.
Targeted transcripts are hybridized to a designed probe and captured with magnetic beads.
The targeted libraries are sequenced by NGS.
A computational pipeline generates an interactive report for rapid insights. All output data files, including gene-cell count matrix, integrate seamlessly with existing open source tools such as Seurat or Scanpy.
Read more about the technology and Immune1000 Gene Panel.
Sequence up to 1 million cells, no instrument required.
Information and insight for the entire range of Parse Biosciences Products.
Reach out for a quote or for help planning your next experiment.
